【英文标准名称】:Indoorair-Part17:Detectionandenumerationofmoulds-Culture-basedmethod(ISO16000-17:2008)
【原文标准名称】:室内空气.第17部分:霉菌的测定与计算.培养基方法(ISO16000-17-2008)
【标准号】:DINISO16000-17-2010
【标准状态】:现行
【国别】:德国
【发布日期】:2010-06-01
【实施或试行日期】:
【发布单位】:德国标准化学会(DE-DIN)
【起草单位】:
【标准类型】:()
【标准水平】:()
【中文主题词】:空气;空气污染;空气净化;空气质量;分析;菌落计数技术;计数技术;培养基;培养(生物);定义;含量测定;过滤;健康危害;保健;杂质;室内气候;室内;内部的空气;记录单;测量;测量技术;微生物分析;霉菌;空气的污染;取样;抽样方法;芽胞;验证;挥发物测定
【英文主题词】:Air;Airpollution;Airpurification;Airquality;Analysis;Colonycounttechniques;Counting-outtechniques;Culturemedia;Cultures(biology);Definitions;Determinationofcontent;Filtration;Healthhazards;Healthprotection;Impurities;Indoorclimate;Indoors;Inneratmosphere;Logsheets;Measurement;Measuringtechniques;Microbiologicalanalysis;Mouldfungi;Pollutionoftheair;Sampling;Samplingmethods;Spores;Verification;Volatilematterdetermination
【摘要】:Mouldsporesarewidelydistributedintheoutdoorenvironmentand,therefore,occurinvaryingconcentrationsalsoindoors.Growthofmouldsinindoorenvironments,however,shouldbeconsideredahygienicproblembecauseepidemiologicalstudieshaverevealedthatdampnessand/ormouldgrowthinhomesandhealthproblemsaffectingtheoccupantsarecloselyrelated.Harmonizedmethodsforsampling,detectionandenumerationofmouldsincludingguidelinesforsamplingstrategiesareimportantforcomparativeassessmentofmouldproblemsindoors.SamplingiscarriedoutaccordingtothemethoddescribedinDINISO16000-16or-18.Agarplates(DG18agarandmalt-extractagarorpotatoextroseagar)obtainedfromsamplingbyimpactionareincubateddirectlyat(25+/-3)?Cand(36+/-2)?C.Filtersobtainedfromsamplingbyfiltrationarere-suspendedinsalinesolution(0,9%NaCl)with0,01%Polysorbate80.DecimaldilutionsofthesuspensionarepreparedandaliquotsspreadonDG18agaraswellasonmalt-extractagarorpotatodextroseagar(indirectmethod).Agarplatesareincubatedat(25+/-3)?C.Forspecialpurposesplatescanbeincubatedat(36+/-2)?C(e.g.thermotolerantAspergillusspp.)or(45+/-2)?C(Aspergillusfumigatus).Afterincubation,mouldcoloniesareidentifiedandcounted.
【中国标准分类号】:C51
【国际标准分类号】:13_040_20
【页数】:29P.;A4
【正文语种】:德语